Signal Transduction Reagents and Kits
Aat Bioquest Calbryte™ 520 AM | 2x50ug
The intracellular calcium flux assay is a widely used method in monitoring signal transduction pathways and high throughput screening of G protein"coupled receptors (GPCRs) and calcium channel targets. Followed by Fluo-3 being introduced in 1989, Fluo-4, Fluo-8 and Cal-520 were later developed with improved signal/background ratio, and became the widely used Ca2+ indicators for confocal microscopy, flow cytometry and high throughput screening applications. However, there are still a few severe problems with Fluo-4. For example, as for Fluo-3, in all most all the intracellular calcium assays with Fluo-4 AM, probenecid is required to prevent the cell-loaded Fluo-4 from leaking out of cells. The use of probenecid with Fluo-4-based calcium assays compromises the assay results since probenecid is well-documented to have a variety of complicated cellular effects. Calbryte™ 520, AM is a novel fluorescent and cell-permeable indicator for the measurement of intracellular calcium.
Aat Bioquest Calbryte™ 520 AM | 1mg
Calbryte™ 520, AM is a novel fluorescent and cell-permeable indicator for the measurement of intracellular calcium. Like other dye AM esters, Calbryte™ 520 AM is non-fluorescent and non-activatable. Once Calbryte™ 520 AM enters the cell, it is readily hydrolyzed by intracellular esterase where it becomes activated and responsive to calcium. The activated indicator is now a polar molecule that is incapable of freely diffusing through the cell membrane, essentially trapping it inside the cell. Upon binding calcium ions, Calbryte™ 520 produces a bright fluorescence signal with extremely high signal/background ratio. It has the identical excitation and emission wavelength as Fluo-4, thus the same Fluo-4 assay settings can be readily applied to Calbryte™ 520-based calcium assays.
Cellecta Inc LentiTrans™ Transduction Reagent (Polybrene for Transduction)
Cellecta’s LentiTrans™ Lentiviral Transduction Reagent is a pre-formulated, easy to use polybrene solution to maximize transduction of any lentivirus. Just add a few microliters to your media during viral inoculation. No preparation necessary. Polybrene is a cationic polymer that enables viral particles to interact more easily with the cell surface. A small amount of the Lenti-Transduction Reagent, which is innocuous to most cell types, into the media during transduction improves rates viral uptake significantly.
Sigma Aldrich Fine Chemicals Biosciences Zolmitriptan Related Compound H United States Pharmacopeia (USP) Reference Standard | 1116-77-4 | MFCD00671479 | 50MG
Zolmitriptan Related Compound H United States Pharmacopeia (USP) Reference Standard | Mol Wt: 189.3 | 1116-77-4 | MFCD00671479 | 50MG
Revvity Health Sciences Inc HTRF Human IL-17A / IL17RA Binding Kit, 10,000 Assay Points
HTRF Human IL-17A / IL17RA Binding Kit, 10,000 Assay Points
![CPC Scientific 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid 1MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00111436-SUBS-016.PNG-250.jpg)
CPC Scientific 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid 1MG
SEQUENCE: 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acidONE-LETTER SEQUENCE: 4-(4-Dimethylaminophenylazo)benzoyl-YVADAPV-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acidMOLECULAR FORMULA: C61H76N12O14S1MOLECULAR WEIGHT: 1233.39STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: [161877-70-9]SYNONYMS: Caspase 1 (ICE) Substrate; dabcyl-yvadapv-edans; dabcyl-tyr-val-ala-asp-ala-pro-val-edans; ICE Substrate Ii; il-1-beta Converting Enzyme Substrate Ii; il-1b Converting Enzyme Substrate Ii; 4-(4-dimethylaminophenylazo)benzoyl-tyr-val-ala-asp-ala-pro-val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid; 4-(4'-dimethylaminophenylazo)benzoyl-tyrosyl-valyl-alanyl-aspartyl-alanyl-prolyl-valyl-5((2-aminoethyl)amino)naphthalene-1-sulfonic acidRESEARCH AREA: ApoptosisREFERENCES:M.W. Pennington et al., Peptide Res., 7, 72 (1994)M. Los et al., Nature 375, 81 (1995)SKU(s): SUBS-016A, SUBS-016B
Echelon Biosciences Research Labs PI(5)P DIC8 AMMONIUM SALT 500 ug
Phosphatidylinositol 5-phosphate diC8 (PI(5)P diC8) is a synthetic, purified dioctanoyl PI(5)P.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 5-phosphate (PI(5)P) is a rare lipid found in the nucleus that can bind the PHD finger of the nuclear adapter protein ING2. PI(5)P is phosphorylated to PI(4,5)P2 by Type II PIP kinases. This item is non returnable
Echelon Biosciences Research Labs 3S-PTDINS(3,4,5)P3 DIC16 500 ug
PtdIns(3,4,5)P3 3-phosphothioate, diC16 (3S-PtdIns(3,4,5)P3, diC16) is a water-soluble, synthetic PI(3,4,5)P3 analog in which the 3-phosphate has been rendered metabolically stable as a phosphorothioate. P-3S908 shows 90% inhibition of PTEN at 0.4M. This item is non returnable
Echelon Biosciences Research Labs EICOSANOYL LPA 1 mg
Arachidoyl LPA, 20:0 LPA (aka Eicosanoyl LPA) is an analog of lysophosphatidic acid (LPA), a lysophospholipid involved in cell proliferation. Lysophosphatidic Acid (LPA) is a small lysophospholipid involved in diverse cellular processes such as cell proliferation, chemotaxis, platelet aggregation, wound healing, angiogenesis, tumor invasion, and smooth muscle contraction. LPA binds to several G-coupled protein receptors to initiate its biological functions. In cancer, LPA primarily promotes cell survival, migration and invasion. May contain up to 5-10% of the 2-acyl isomer due to acyl transfer. This item is non returnable
Carnabio Usa Inc CK1G1(CSNK1G1)/10UG/FULL-LENGT
CK11(CSNK1G1), Full-length, Wild type, Amino Acid 1-422, NP_071331.2, Expressed in Insect (sf21); 10microg
Revvity Health Sciences Inc HTRF cAMP Gs HiRange Detection Kit Standard, 10 Vials
HTRF cAMP Gs HiRange Detection Kit Standard, 10 Vials